Amplified Fragment
Length Polymorphism (AFLP) analysis in Cyamopsis spp. for Genetic Diversity Study
Bhatele Pranita, Dhawan Sunita Singh, Aswathnarayana Nagesh K., Dixit Shikha and
Randhawa Gursharn Singh
Res. J. Biotech.; Vol. 17(1); 143-148;
doi: https://doi.org/10.25303/1701rjbt143148; (2022)
Abstract
The aim of present work is to study genetic diversity and phylogenetic relationship
among 13 guar varieties from different germplasm conservation centers of India.
A total of 64 primer combinations were tested and 24 primer combinations producing
best DNA fingerprints were selected for final analysis. The AFLP marker gives keen
amplification with 1,671 polymorphic bands and 62 unique bands ranging from 55 bp
to 350 bp. Maximum numbers of unique bands were produced with E-ACT/M-CAG primer
combination.
This analysis allows determining association with an undefined feature and helps
in identification of these genotypes. The phylogenetic relationship was explained
by a dendrogram generated with UPGMA method. These guar genotypes may be used to
develop new varieties with significant improvement in quality and quantity of gum.
