CYP3A4*2 Gene
Polymorphism and its association with Clinical Phenotyping (temperament) Concept
of Unani Medicine Philosophy in Indian Population
Pallapolu Priyanka, Ghali Shravan Kumar, Ghazala Javed, Kazmi Munawwar Husain and
Chakraborty Alokananda
Res. J. Biotech.; Vol. 17(1); 52-59;
doi: https://doi.org/10.25303/1701rjbt5259; (2022)
Abstract
Phenotypic heterogeneity is one of the major concerns in personalized medicine.
Variations in drug metabolism rates vary in every individual and have been associated
with drug metabolizing gene polymorphism. CYP3A4*2 gene polymorphism in drug metabolism
has been reported to be associated with a range of diseases. In this regard, the
present investigation is aimed to determine the possible association of Unani philosophy
of temperaments (clinical phenotypes) with CYP3A4*2 gene polymorphism in patients
and controls. In the present study, 800 subjects were analyzed for CYP3A4*2 gene
polymorphism by PCR-RFLP technique in four different clinical phenotypes (Sanguine,
Phlegmatic, Choleric and Melancholic) as per unani philosophy.
Mutant allelic frequencies of patients and controls of four unani temperaments are
sanguine (Damavi) (0.19 vs 0.12), phlegmatic (Balghami) (0.21 vs 0.11), bilious
(Safravi) (0.23 vs 0.11) and melancholic (Saudavi) (0.16 vs 0.11) subjects. Safravi
(p<0.04) and Balghami (p<0.01) results are statistically significant. Bilious (Safravi)
and phlegmatic (Balghami) patients showed significant association with CYP3A4*2
gene polymorphism with 1.76 and 2.04 odds risk. Further such studies are essential
in unani regime for providing better treatment for many diseases which require personalized
medicine based on phenotype-genotype correlation.
