Evaluating in-vitro
antioxidant and in-silico anti-inflammatory potential of Diosgenin
Manobharathi V., Kalaiyarasi D. and Mirunalini S.
Res. J. Biotech.; Vol. 17(1); 111-118;
doi: https://doi.org/10.25303/1701rjbt111118; (2022)
Abstract
Chronic diseases are inextricably tied up with oxidative stress and inflammatory
responses. Oxidative stress triggers an antioxidant imbalance that elicits a multitude
of inflammatory reactions, inevitably culminating in death-causing diseases by integrating
free radicals. Rising research approaches intensely focus on identifying natural
antioxidants with efficient anti-inflammatory potency. Diosgenin is a potent bioactive
therapeutic candidate spotted in Fenugreek and Yams and has varied pharmacological
properties. Concerning the therapeutic importance of this compound, the current
study intends to explore its antioxidant and anti-inflammatory potential. The antioxidant
ability of diosgenin was tested by using free radical scavenging assays such as
1,1-diphenyl-2- picrylhydrazyl radical (DPPH), 2, 2’-Azinobis-(3-ethyl benzothiazoline-6-sulfonic
acid) (ABTS●+), Hydroxyl radical and Superoxide anion. Furthermore, the screening
of Diosgenin’s anti-inflammatory potential was done by ligand interactions via Autodock
software to explore its binding affinity with inflammatory receptors (NF-κB and
TNF-α). On comparing this with standard ascorbic acid, Diosgenin has nearly equivalent
IC50 values.
The IC50 values of Diosgenin and Ascorbic acid were found to be 48.24 μg/mL and
47.93 μg/mL (DPPH), 41.18 μg/mL and 40.19 μg/mL (ABTS●+), 50.14 μg/mL and 48.99
μg/mL (Hydroxyl radical), 44.33 μg/mL and 50.02 μg/mL (Superoxide anion). Additionally,
Diosgenin has good binding scores (-7.7, -9.8 kcal/mol) with target inflammatory
receptors (NF-κB and TNF-α). In-vitro and in-silico modeling proves that diosgenin
has effectively high antioxidant and antiinflammatory potency. Hereby, diosgenin
seems to be a promising antioxidant against inflammatory diseases.
