Isolation, characterization, antibacterial properties of a tilapia autochthonous probiotic bacteria and in vivo protective response of tilapia fed with the tilapia isolate

Azad I. S. and Ridha M. T.

Nile tilapia from the aquaculture farms of Kuwait and from the aquaculture faculties of Kuwait Institute for Scientific Research were used for isolating autochthonous gut bacteria for applications as in-feed probiotics in aquaculture of this species. A total of 36 isolates were obtained of which 10 isolates were preliminarily differentiated for characterization through API 20-E and 16s rDNA-based gene sequence analysis. Anti bacterial activity determined through over-lay method showed that only one tilapia isolate (TPI) was producing antibacterial effect on three of the four pathogenic bacteria tested. The wound healing of the TPI injected fish was faster and the isolate was identified to be highly similar to Bacillus subtilis strain DP12 (GenBank Accession Number : HQ536001.1) based on nucleotide homology and phylogenetic analysis. The RPS (75 to 81) of Nile tilapia fed autochthonous probiotic (TP1) was highly significant reflecting on the potential benefits of the autochthonous probiotics. In the light of several tests, the potential benefits of this isolate have been discussed.

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Isolation, identification and analysis of DDT degrading bacteria for agriculture area improvements

Powthong Pannapa, Jantrapanukorn Bajaree and Suntornthiticharoen Pattra

Dichlorodiphenyltrichloroethane (DDT) is an organochlorine pesticide that can persist in the environment resulting in environmental problem with chronic effects on human and animal health. Biodegradation is a potential method to detoxify the recalcitrant compounds. In this study, the potential for biodegradation of DDT through enrichment and isolation of DDT degrading bacteria from soil and water in agricultural area was done. Microorganisms grew in minimal media with DDT as the only carbon source. Six from 40 bacteria isolated were selected by increasing concentrations of DDT. The DDT degradation was quantified by the enrichment cultures places degrading S4-67.95%, S13-65.05%, S37-65.45%, S39-67.25%, W30-65.20% and W37- 67.55% of the initial amount of DDT respectively in 7 days. Further, the optimal of temperature, pH and salinity of the isolate has also been investigated. Promising DDT degrading isolates were tentatively identified by biochemical and MALDI-TOF MS characterization as 3 groups: Pseudomonas stutzeri (3 isolates), Pseudomonas aeruginosa (2 isolates) and Bacillus firmus (1 isolate).

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Evaluation of Corrosion Inhibition of Sida acuta Leaves Extract on Carbon Steel immersed in Acid Medium

Hema K., Venkatraman B. R. and Subramania A.

The corrosion inhibition of leaves extract of Sida acuta was investigated by evaluating the corrosion behaviour of carbon steel immersed in 1N HCl solution containing various concentrations of the extract. Mass loss, corrosion rate and adsorption characterization were utilized to evaluate the corrosion inhibition and adsorption properties of the extract. The results obtained from weight loss studies revealed that the Sida acuta leaves extract is an efficient inhibitor at the best concentration of 800ppm with maximum inhibition efficiency (90.3%) on carbon steel immersed in 1N HCl solution. The corrosion rate was observed to decrease with increase in concentrations of extract but increase with temperature. The activation energy and the negative free energy of adsorption obtained from the adsorption studies indicate that the Sida acuta leaves extract is physically adsorbed on the surface of the carbon steel and the adsorption is strong, spontaneous and fits with the assumption of the Langmuir adsorption isotherm.

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Will Silver Barb (Barbonymus Gonionotus) survive at Floating Net Cage in Cirata Reservoir by Gulma Water Hyacinth (Eichhornia Crassipes) alone?

Rostika R., Yustiati A., Herawati T. and Ahadian I.

This research aims to identify the average daily gain (ADG) and survival rate (SR) of silver barb fed with water hyacinth farmed at floating net cage in Cirata Reservoir. Experiment is conducted from December 2014 to February 2015 using complete random model consisting of four treatments and three repetitions. The treatments include water hyacinth feeding for 10%, 20% and 30% and artificial feeding for 3% of the fish's weight. Fish are fed two times a day by observing the ADG, SR and water quality. The results show that water hyacinth feeding greatly influences the ADG; the 3% feeding supplies the highest ADG by 1%, while the other treatments equally contribute 0.1%. However, there is no significant difference in the average survival rate of the fish resulting in 98%.

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Equilibrium and kinetic studies of the adsorption of congo red onto modified fly ash

Ebelegi Newton Augustus, Ayawei Nimibofa and Wankasi Donbebe

Fly ash was modified by chemical treatment with NaOH solutions of varying concentrations (4M, 3M, 2M, 1M) and labeled SHFA-4, SHFA-3, SHFA-2, SHFA-1 respectively. All four forms of fly ash were tested as adsorbents for the removal of Congo Red dye from aqueous solutions. The effects of contact time and concentration were investigated using a batch adsorption technique. The experimental data could best be described by the Freundlich isotherm and the dynamic data fit well with the pseudo-second-order kinetic model which suggests that the adsorptions appeared to be controlled by chemisorption processes. Fly ash modified with 4M NaOH (SHFA-4) demonstrated the highest adsorption capacity (KF =3.322mg/L). The Gibbs free energy of adsorption ( ∆G_ads^O) values were: SHFA-4 (-2884.4Jmol-1), SHFA-3 (-547. 16Jmol-1), SHFA-2(-1961.4Jmol-1), SHFA-1 (-909.4Jmol-1) which show the adsorption processes were spontaneous. These results suggests that fly modified with NaOH could be employed as a low cost adsorbent for the removal of Congo Red from waste water streams from textile, tanning and printing industries.

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Bioremediation of Chromium

Hazarika Samiul Islam

In the present study, biosorption of Chromium by microorganisms from Coal mine effluent was investigated. The initial concentration of heavy metal Chromium (K2Cr2O7) in the effluent was 120mg/lit. The bacteria isolated from industrial effluent were identified as Bacillus megaterium and Pseudomonas aeruginosa by biochemical and 16S rDNA sequencing. The effect of pH and temperature on the biosorption capacity was investigated. Under the optimum conditions the highest uptake in pH 7 at 300C was 96% and 90% in case of Bacillus megaterium and 95 and 90% in case of Pseudomonas aeruginosa. At low temperature and low pH the efficiency was reduced.

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Yield and biochemical attributes of some mango (Mangifera indica L.) hybrids under agro-climatic conditions of Bihar

Karuna K., Kumar M. and Mankar Abhay

The experiment was conducted in the experimental orchard of the Department of Horticulture (Fruits and Fruit Tech.) at Bihar Agricultural College, Sabour during the year 2011. Eleven mango hybrids (Alfazli, Amrapali, Jawahar, Mahmoodbahar, Mallika, Neeleshan, Neeludin, Prabhashankar, Ratna, Sabri and Sundar Langra) as well as the most important variety of this region Langra as check were used to conduct this experiment. Morphological, biochemical and yield attributes were studied to evaluate them. The hybrid Amrapali gave better performance in respect of TSS and total sugars content i.e. 24.64ºBrix and 15.32% respectively in fruits while Mallika was better in acidity (0.356%) and it also produced heaviest fruits (604.10g) with higher yield (151.21 kg/plant). So far the pulp percentage and ascorbic acid are concerned, the check variety Langra (76.04% and 118.32 mg/100g juice respectively) proved better in comparison to the hybrids under study. Pulp quality was found also good in Langra fruits. Many of the hybrids performed well over the Langra in terms of different aspects.

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