Reverse transcription
PCR-based detection of matrix and hemagglutinin-neuraminidase genes among Avian
orthoavulavirus 1 clinical isolates in the Philippines, 1991-2017
Tolentino Jarel Elgin M., Angeles Jorge Gil C., Galay Remil L. and Umali Dennis
V.
Res. J. Chem. Environ; Vol. 25(9); 89-95;
doi: https://doi.org/10.25303/259rjce8995; (2021)
Abstract
Newcastle disease (ND) is a highly infectious disease that affects devastatingly
the avian population worldwide. It is caused by Avian orthoavulavirus 1 (AOAV-1),
or better known as Newcastle disease virus belonging to phylum Negarnaviricota,
class Monjiviricetes, order Mononegavirales and family Paramyxoviridae. This virus
consists of six principal structural proteins namely: the nucleocapsid protein (NP),
phosphoprotein (P), matrix protein (M), fusion protein (F), hemagglutinin-neuraminidase
protein (HN) and the large protein RNA dependent RNA polymerase (L)..
The present study aimed to molecularly detect the M and HN gene segments of the
AOAV-1 field isolates from clinical cases in the Philippines from 1991 through 2017.
RT-PCR amplification and sequence analyses using primers NDV-For4359 and NDV-Rev4788
which anneal to the matrix gene and primers NDV-For6369 and NDV-Rev6598 targeting
the HN genes, identified all isolates to be AOAV-1. Determining the different genes
of the virus would greatly help scientists and researchers to accurately identify
the viral isolates in order to improve epidemiological studies and surveillance
of the disease in the country.
