Chemical components
as a potential resource for synthesis of organic compounds and anti-inflammatory
activity of essential bark oil of Illicium verum
Van Dinh Thuy, Chinh Pham The, Tham Pham Thi, Nga Mai Thanh and Ounkuea Thongsaving
Res. J. Chem. Environ.; Vol. 28(1); 68-72;
doi: https://doi.org/10.25303/281rjce68072; (2024)
Abstract
In this work, the essential bark oil (EBO) of Illicium verum cultivated at Cao Bang
in Vietnam obtained by hydro-distillation was investigated for its chemical composition
by gas chromatography-mass spectrometry (GC-MS). Trans-anethole (78.6%), estragole
(13.7%), linalool (2.1%) and eucalyptol (2.0%) were determined to be the main components
among 14 identified compounds, which accounted for 96.4% of the total EBO. Anti-inflammatory
activity of EBO was evaluated by inhibition of nitric oxide (NO) production in lipopolysaccharide
(LPS)-stimulated RAW 264.7 cells.
EBO exhibited an interesting inhibitory effect on NO production in LPS-stimulated
RAW246.7 cells with an inhibitory value at 93.87 μg/mL. From the starting material,
trans-anethole isolated from EBO, there are two new amine-derivatives that were
synthesized. The structure elucidation of these analogues of trans-anethole was
carried out by 1D (1H NMR and 13C NMR) and IR spectral analysis.
