Exploring the
therapeutic potential of Adathoda vasica and Andrographis paniculata in alleviating
diabetic cardiopathy and neuropathy: A study on high-fat diet and low-dose STZ-induced
rat model
Kavya Manjunath, Hariprasad Madakasira Guggilla and Purnima Ashok
Res. J. Chem. Environ.; Vol. 28(6); 9-15;
doi: https://doi.org/10.25303/286rjce09015; (2024)
Abstract
Diabetes mellitus is the metabolic illness, if it is left untreated it leads to
secondary complications especially in patients suffering from T2DM. Herbal drugs
are in medical use since ages. It is found that both Adathoda vasica and Andrographis
paniculata exhibited anti-diabetic activity. Therefore, this study is aimed to explore
their therapeutic effects in alleviating the secondary complications like neuropathy,
cardiopathy using HFD-low dose STZ experimental model. Present study is designed
to explore the therapeutic potential of an aqueous ethanolic extract of Adathoda
vasica and Andrographis paniculata in alleviating diabetic cardiopathy and neuropathy
against HFD-low dose of STZ induced diabetic model.
Diabetes was induced by treating the animals with HFD-low dose of STZ injection.
Diabetic animals were selected for further investigation on cardiopathy and neuropathy
where untreated and treated (Pioglitazone, increasing doses of AEEAV, AEEAP) diabetic
animals were subjected to Eddy’s hot plate test, Tail immersion test and Charcoal
meal test. Oxidative parameters (MDA, GSH, SOD, CAT) were measured from sciatic
nerve and heart homogenate, serum CK-MB, LDH, TC, TG, LDL and HDL were also measured.
AEEAP showed improvement in intestinal transit time (P<0.001), restored antioxidants
and reduced MDA levels in comparison with untreated diabetic animals (P<0.001).
Cardiac biomarkers like CK-MB, LDH, TC, TG, LDL were significantly reduced in dose
dependent manner (P<0.001) whereas HDL was found to be increased in treated diabetic
animals. In addition, histopathology studies were well co-related with bio chemical
improvement. Therefore, aqueous ethanolic extract of Adathoda vasica and Andrographis
paniculata alleviates diabetic cardiopathy and neuropathy in HFD- STZ model of diabetes.
