Effect of Radiation
on Oral Cancer patients by 1H NMR based Metabolomic
Maurya Reeta, Sen Manodeep, Rastogi Madhup, Singh Alok Kumar and Roy Raja
Res. J. Biotech.; Vol. 20(4); 177-183;
doi: https://doi.org/10.25303/204rjbt1770183; (2025)
Abstract
Oral microflora is well-orchestrated and acts as an in-order defense mechanism for
any infection related to oral disease. It has an encouraging outcome in terms of
early recovery biomarkers. The saliva of oral cancer patients' subjects and controls
have been evaluated by 1 H NMR spectroscopy in search of possible early metabolic
differences that could be obtained to see the eradication of disease that favors
the symbiotic condition. The study employed 1 H NMR spectroscopy on 102 human saliva
samples (including case and controls) and their spectral data were further subjected
to multivariate and quantitative analysis.
The 1H NMR study of oral cancer samples shows clear demarcation and profound metabolic
differences when compared with the controls. Several metabolites such as lactate,
ethanol, succinate and glutamate were found to be of higher significance in oral
cancer patients in contrast to controls. Significant metabolites could be considered
as early repair markers for oral cancer patients as they are being restored to achieve
symbiosis. The study, therefore, concluded the early recovery process of the diseased
subjects with the restoration of possible metabolomic profiles similar to the controls.
