Formulation and
Evaluation of Phytosome containing Trigonella foenum graecum Extract for in vitro
Antioxidant and Anti-inflammatory Potential
Nayak Smita S., Patil Akshay K., Patil Shreya, Deshpaik Saba and Gudasi Sachin
Res. J. Biotech.; Vol. 20(4); 10-17;
doi: https://doi.org/10.25303/204rjbt010017; (2025)
Abstract
The objective of current research work was to formulate Trigonella foenum graecum
extract loaded phytosome and to evaluate its in vitro antioxidant and anti-inflammatory
potential. The plant material was analysed for its quality and subjected for extraction.
The extract was subjected for phytochemical investigations. Compatibility of drug
and excipients was confirmed by FTIR. Phytosomes were prepared by thin film hydration
method. The phytosomes were analysed for particle size and polydispersability index.
The optimized formulation (TFG4) was subjected for in vitro antioxidant and anti-inflammatory
assays.
All physicochemical parameters were found to be within limit. The phytochemical
investigation confirmed the presence of alkaloids, glycosides, steroids, terpenoids
and phenols. The compatibility study confirms the compatibility of excipients and
drug. Batch 4 (TFG4) exhibited smaller particle size of 244 nm and PDI of 0.5. With
an IC50 value of 54.17 μg/ml, it is proven to have beneficial effects as antioxidant
which was compared with standard ascorbic acid with an IC50 of 34.50μg/ml. In vitro
anti-inflammatory activity showed an IC50 value of 56.68 μg/ml for sample and 28.63
μg/ml for standard. It can be concluded that phytosomes loaded with Trigonella foenum
graecum exhibited promising antioxidant and anti-inflammatory potential and can
be used as treatment model with better therapeutic efficiency.
