Biotechnological
potentials of Selinium nanoparticle from E-scrap by Bacillus subtilis
Ramla Asmi M., Jeevitha V. and Udhayaraja P.
Res. J. Biotech.; Vol. 20(8); 226-231;
doi: https://doi.org/10.25303/208rjbt2260231; (2025)
Abstract
Microorganism isolation from contaminated sites provides unique properties useful
for bioremediation, particularly in managing electronic waste (E-waste). This study
focused on isolating selenium-resistant bacteria from the photocopier drum of E-waste
collected from a dumpsite. Two strains, labeled S1 and S2, were isolated, with the
S2 strain demonstrating selenium tolerance. On selective agar plates, the isolated
strain produced small, orange to red-orange colonies. Selenium nanoparticles (SeNPs)
were biologically synthesized by Bacillus sp. and characterized using UV and SEM
analysis. Reduction was observed at 6 hours, with significant color changes at 24
hours. A sharp absorption peak was detected at 280 nm, likely due to surface plasmon
vibrations.
The synthesized SeNPs were rod-shaped, measuring 50 to 100 nm. Pathogens isolated
from wounds were found to be sensitive to the biosynthesized SeNPs. Molecular identification
through neighbor-joining phylogenetic analysis identified the S2 strain as Bacillus
cereus. Therefore, biosynthetic SeNPs show potential as an alternative to traditional
antibiotics, reducing side effects and enhancing therapeutic efficiency. However,
further analysis and in vivo testing are needed to explore various aspects of using
SeNPs.
