The association
of trehalose and DMSO in cryopreservation of mouse cauda epididymal spermatozoa
Ha L.B.T., Hoang M.L. and Tuyet T.V.L.
Res. J. Biotech.; Vol. 20(8); 160-166;
doi: https://doi.org/10.25303/208rjbt1600166; (2025)
Abstract
It has been shown that mouse sperm is more challenging to cryopreserve than the
sperm of other mammals. The association of nonpermeating agents with penetrating
agents reduces the concentration of penetrating agents, reducing their toxicity.
This study evaluated the effect of the association of different concentrations of
trehalose (0, 0.05 M, 0.1 M and 0.15 M) with 2.5% DMSO in the presence of 5 mg/mL
human serum albumin (HSA). Accordingly, mouse spermatozoa samples collected directly
into each CPA group were frozen/thawed under identical conditions. The assessments
including viability, motility and DNA fragmentation rate were performed after one
day, three days and seven days of freezing. Moreover, the fertilization function
of spermatozoa in the group that maintained the best characteristics after cryopreservation
was evaluated.
The results showed that a higher percentage of the parameters including viability,
motility and DNA integrity rates were observed in the 0.1 M trehalose group. The
in vitro fertilization results demonstrated that mouse spermatozoa frozen with the
0.1 M trehalose group were less fertile than those with fresh spermatozoa (p < 0.05).
According to our research, the ideal parameters for cryopreservation were obtained
when the cryopreserve medium contained 0.1 M trehalose in combination with DMSO.
