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Extended-Spectrum β-Lactamase (ESBL) Genotypes among Multidrug-Resistant Uropathogenic Escherichia coli from Clinical Isolates

Saran Kumar S. and Lali Growther

Res. J. Biotech.; Vol. 20(12); 221-227; doi: https://doi.org/10.25303/2012rjbt2210227; (2025)

Abstract
Urinary tract infections (UTIs) are a major public health concern with Escherichia coli being the predominant uropathogen. The increasing emergence of multidrug-resistant (MDR) strains, particularly those producing extended-spectrum β-lactamases (ESBLs), complicates treatment strategies. This study aimed to detect and characterize ESBL genotypes among MDR uropathogenic E. coli isolated from clinical urine samples. Phenotypic confirmation of ESBL production was performed using standard disc diffusion methods. Molecular analysis was conducted to identify the presence of key ESBL genes including bla_TEM, bla_SHV and bla_CTX-M.

A high prevalence of ESBL producers was observed with bla_CTX-M being the most common genotype detected. The isolates exhibited resistance to multiple antibiotic classes, while showing susceptibility to carbapenems. The findings highlight the urgent need for molecular surveillance and antibiotic stewardship to curb the spread of ESBL-producing uropathogens in clinical settings.