Evaluation of
ACC Deaminase producing bacteria over non-producers in protecting Vigna radiata
growth under Salt and Boron stress
Landge Vishal Rajendra and Patil Niranjan Prakashrao
Res. J. Biotech.; Vol. 20(7); 81-90;
doi: https://doi.org/10.25303/207rjbt081090; (2025)
Abstract
The 1-aminocyclopropane-1-carboxylate deaminase (ACCD) is a crucial bacterial trait
found in rhizobacteria for amelioration of different plant stresses. A co-inoculation
approach is explored in this study. Our objective was to compare the efficacy of
PGPB with ACCD activity and without ACCD activity in improving the growth of legume
Vigna radiata under salt and boron stress. Two isolates were selected for comparative
study in the mitigation of salt and boron stress with and without ACCD activity.
Seeds of Vigna radiata were co-inoculated with TKP-A3 having ACCD activity and BVB-4
lacking ACCD exposed with two different concentrations of boron (6.5mM and 7.5mM
H3BO3) under saline stress (40 mM NaCl). Our findings confirm that the detrimental
effects due to excess salt and boron were successfully mitigated in plants co-inoculated
with the TKP-A3 as compared to BVB-4. Our results have shown that salt and boron
stress significantly altered the lateral root formation, root length, chlorophyll
content and vigor index of the seedlings.
Interestingly, the addition of TKP-A3 significantly increased shoot length, vigor
index and chlorophyll content by almost 15%, 16% and 50% respectively compared to
BVB-4, indicating the importance of ACCD activity in the mitigation of salt and
boron stress. Additionally, no positive effects were observed on different growth
parameters when seeds were coated with BVB-4 alone. This study highlights the significance
of ACCD producing PGPB over PGPB lacking ACCD to improve growth characteristics
of Vigna radiata under salt and boron stress. Molecular analysis identified TKP-A3
and BVB-4 as Enterobacter quasiroggenkampii and Cytobacillus pseudoceanisediminis
respectively.
