In vitro propagation
of Homalomane occulta, A medicinal herb from Vietnam
Nhung Dang Thi, Thao Nguyen Huynh Yen, Minh Nguyen Hoang Nhat, Thanh Pham and Trang
Nguyen Thi Quynh
Res. J. Biotech.; Vol. 20(7); 1-8;
doi: https://doi.org/10.25303/207rjbt0108; (2025)
Abstract
Homalomane occulta (H. occulta) is an herbal plant with many great traditional medicinal
values. In general, H. occulta is mainly propagated naturally from seeds or rhizomes,
with a relatively low multiplication rate. They also have slow growth rates and
depend highly on seasonal and weather conditions. Propagation using tissue culture
was reported to have many advantages compared with traditional propagation methods.
This research aims to study the in vitro propagation of H. occulta, a medicinal
herb from Vietnam. Our study showed that the rhizome section carrying the bud is
the most effective culture organ, sterilized with 0.1% HgCl2 for 8 minutes, which
has an excellent disinfection effect and a high survival rate (82.87%). The suitable
medium for in vitro shoot regeneration of H. occulta is MS medium with sucrose (30
g/L) and agar (8 g/L) supplemented with 4.0 mg/L BAP (2.10 shoots/explant).
The appropriate medium for rapid multiplication and formation of shoot clusters
and roots is essential MS medium supplemented with 1.0 mg/L TDZ (with 5.20 shoots/explant,
9.40 roots/shoot clumps). When transferred from an in vitro environment to an open
garden, seedlings have a 100% survival rate.
