Molecular Characterization
and Phylogenetic Analysis of Multidrug Resistant Microorganism with Special Reference
to Nosocomial Infections
Khajuria Komal, Sharma Neha and Tomar Rajesh Singh
Res. J. Biotech.; Vol. 20(7); 74-80;
doi: https://doi.org/10.25303/207rjbt074080; (2025)
Abstract
The bacterial isolates from hospital environment act as a pool of antimicrobial
resistance as well as main cause of nosocomial infections and their genes act a
potential source of resistance to clinical pathogens. In the present study, we aim
to perform molecular characterization of bacteria from hospital settings and to
identify the emerging multi-drug resistant bacteria in our study area. A study was
carried out in various hospitals in Gwalior, Madhya Pradesh, India. Collection of
isolates was carried out, transported and immediately analyzed by using standard
protocols. Identification of bacterial isolates was done using biochemical tests
and 16S rDNA sequencing technique. Mueller-Hinton agar was used for carrying out
antibiotic susceptibility test against beta-lactam group of antibiotics using Kirby-Bauer
disk diffusion method.
Amplification of DNA was done was using polymerase chain reaction with the help
of universal primers. A total of 46 bacterial isolates were found from different
areas of various hospitals. Four isolates were picked for molecular characterization
and phylogenetic tree construction using distance matrix method. We found that three
out of four belong to Staphylococii group whereas one is from Bacilli group. In
conclusion, we have found 4 prominent bacterial strains as MDR against beta-lactam
group of antibiotics and it was observed that variations in the sequences of target
bacterial species can be attributed to provide resistance to different antibiotics
and gene transfer among bacterial strains.
