Research Journal of Biotechnology

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Cytogenetic effects of gamma radiation-induced seed extracts of Panicum miliaceum L. on the somatic chromosomes of Allium cepa L.

Amitha Nanggia R. and Manimozhi V.

Res. J. Biotech.; Vol. 20(6); 25-30; doi: https://doi.org/10.25303/206rjbt025030; (2025)

Abstract
Studies on the interaction between biological systems and Gamma radiation have been used as an efficient approach for determining both its beneficial and damaging effects. The present study was carried out to assess the induced Gamma irradiation effects on cytogenetic parameters in proso millet (Panicum miliaceum L.) variety CO(PV) 5 by exposing the seeds to Gamma source - Cobalt-60 (60Co) at doses ranging from 50, 100, 150 and 200 Gy.

The results of cytological studies with Allium cepa bioassay revealed a wide range of chromosomal aberrations viz. stickiness, bridges, laggards, fragments, nuclear lesions, binucleated cells, precession, promiscuous chromosomes, disoriented oblique spindles and chromosome degeneration. The study concluded that the results were concentration-dependent, time-dependent and Gamma-dose-dependent.