Research Journal of Biotechnology

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In vitro propagation, Antibacterial and Antioxidant activities of Coleus Zeylanicus (Benth.) L.H. Cramer

Kanagaraj K., Nandagopal S. and Ganesh Kumar A.

Res. J. Biotech.; Vol. 20(6); 137-146; doi: https://doi.org/10.25303/206rjbt1370146; (2025)

Abstract
The present study is an in vitro micropropagation of Coleus zeylanicus, a potential therapeutic plant utilizing shoot tip and nodal explants by following plant tissue culture methods. BAP and KIN blends brought about a normal shoot length of 4cm per explant. This blend likewise delivered 13 shoots most extreme for each explant with 90% shooting proficiency. Numerous shoots raised were additionally stretched on MS medium containing IAA (0.5-2.0 mg/ml) and IBA (0.5-2.0 mg/ml).

Established plantlets were then moved to plastic pots containing autoclaved garden soil, yard soil and sand in the proportion of (1:2:1) for solidifying measure (hardening). The endurance pace of plantlets changed under acclimatization and plants looked sound with no noticeable distinguishable phenotypic varieties. This is the primary report on plant recovery through organogenesis of Coleus zeylanicus. The examination likewise presents accessible information for all out phenolic and flavonoid content, its antioxidant and antibacterial action against five bacterial strains of in vivo and in vitro plant.