Isolation and
molecular identification of keratinase hydrolyzing microorganisms isolated from
feather samples of Gulbarga region
Hema R., Lingappa K. and Sindhe Ananda
Res. J. Biotech.; Vol. 20(6); 6-10;
doi: https://doi.org/10.25303/206rjbt06010; (2025)
Abstract
Enzymes are delicate protein molecules necessary for life. Among various different
enzymes, protease are the enzymes which are found to play an important role in the
metabolism of almost all organisms. Among proteases, Keratinase (EC 3.4.4.25) is
able to hydrolyze insoluble keratins more efficiently than other proteases and their
action are very specific i.e. they act only on keratin substrates. Keratins are
insoluble fibrous proteins found in hair, wool, feather, nail, horns and other epithelial
covering which are rich in beta helical coil linked through cysteine bridges. Keratinases
are widely used not only in chemical and medical industries but also in food in
dustries.
In respect to this, present study aimed to isolate keratin hydrolyzing micro-organisms
from the feathers samples collected from various butcher shops and poultry farm
in and around Kalyan-Karnataka region where we have isolated around 46 isolates
from 20 different types of samples among which KLHR-30 was found to be potent keratinase
producer showing activity of 90 U/ml found to be an actinomycetes from molecular
analysis and deposited in gene bank with an accession number of OR841343.
