Prevention of
Browning during in vitro Cultures of Verbascum scamandri
Cambaz E. and Çördük N.
Res. J. Biotech.; Vol. 20(6); 42-47;
doi: https://doi.org/10.25303/206rjbt042047; (2025)
Abstract
Browning, a common problem in plant tissue cultures, can hinder callus production
and the synthesis of secondary metabolites. This study investigates browning in
the in vitro callus culture of Verbascum scamandri, a plant species with significant
medicinal potential. This study aims to evaluate the efficacy of various browning
inhibitors. In the study, leaf explants from 15-week-old plants grown in vitro were
used to investigate browning in callus cultures. Explants were cultured in MS basal
medium supplemented with 3% sucrose, 0.7% phyto agar and 2 mg/L Kinetin, which was
chosen for its high callus proliferation. Treatments included various concentrations
(50, 100, 200 and 300 mg/L) and combinations of browning inhibitors (PEG, PVP, AA,
CA, AC and AA+CA), as well as dark culture conditions. The density of browning and
callus was recorded and calculated after 28 days including texture and color data
of induced calli.
According to the results, it was found that the most effective treatment for the
prevention of browning is culturing in dark conditions. Additionally, it was observed
that in this treatment, the density of callus induction was higher and the induced
calli did not brown and had a compact texture.
