Report on Characterization
and Antimicrobial Activity of Biogenic Synthesis of Silver Nanoparticles from Amchur
(Indian Spice) obtained from Mangifera indica: A Sustainable Resource
Tailor Shalini, Malik Ayushi, Gaur R.K. and Marwal Avinash
Res. J. Biotech.; Vol. 20(6); 126-136;
doi: https://doi.org/10.25303/206rjbt1260136; (2025)
Abstract
Rapid development and advancements in nanotechnology with its green synthesis approach
for nanoparticle preparation have led to many opportunities for research. This study
discusses the synthesis of silver nanoparticles (SNPs) from the aqueous extracts
(AE) of dried mango fruit (Amchur), an Indian spice prepared from Mangifera indica.
Green synthesis of SNP was done by preparing an AE of Amchur mixed with 1 mM AgNO3.
The characterization of the synthesized SNP was done by observing the color change
from light yellow to deep brown and with UV-VIS spectrophotometry from the 300-700
nm range. Further characterizations were done using X-ray diffraction (XRD), Scanning
electron microscopy (SEM), Energy-dispersive X-ray spectroscopy (EDX) and Fourier
transform infrared spectroscopy (FTIR). The phytochemical tests were performed from
the crude AE of amchur proving the presence of several plant secondary metabolites
like phenolic compounds, saponins and sugars. The antimicrobial activity (AMA) of
Amchur SNP (ASNP) was detected using the disc-diffusion method against Gram-positive,
Gram-negative bacteria and Candida species. Statistical analysis was done using
a single-factor ANOVA.
t-tests were used to compare populations of interest and statistical significance
was defined as p < 0.05. The average size of the green synthesized SNPs was ̴10
nm and cubic. The absorbance spectrum was observed at the peak of 440 nm in UV-Vis
spectroscopy. The antibacterial activity was found to be more potent than the antifungal
activity provided by the SNPs. The inhibitory effect of the ASNP is satisfactory
in comparison to the standard drugs. The current study sought to create a novel,
economical, environmentally benign method for plant-mediated SNP production and
its antimicrobial efficacy.
