An efficient micropropagation
protocol for an economically valuable medicinal plant Kaempferia parviflora Wall.
Ex Baker
Barvaliya Parita, Patil Ghanshyam, Phurailatpam Arunkumar, Patel Ankit and Mankad
Mounil
Res. J. Biotech.; Vol. 20(3); 138-149;
doi: https://doi.org/10.25303/203rjbt1380149; (2025)
Abstract
Kampferia parviflora is an important medicinal plant that is used in many popular
systems of traditional medicine. Fresh plant material for medicinal uses and research
purposes is critically unavailable around the year due to its long dormancy period.
This study aimed to establish an aseptic culture of K. parviflora using silver nanoparticles.
Explants from rhizome buds were cultured on Murashige and Skoog (MS) medium supplemented
with different concentrations of individual plant growth hormone BAP, Kinetin, ADS
and 2ip. Highest number of shoots was 6.33, highest multiplication rate was 3.33,
highest number of leaves was 3.33 recorded at 6 mgl-1 BAP and highest length of
shoots 2.7 were recorded on 8 mgl-1 BAP. When explants were inoculated on MS medium
containing different concentrations and combinations of BAP with NAA, IAA, Kinetin
and ADS, highest number of shoots was 5.67.
Thus, individual effects of BAP improved significantly the shoot growth and proliferation.
MS medium supplemented with half strength MS media with 2 mgl-1 NAA and gave the
highest number of roots (7.56). However, longest roots per explant were obtained
with MS + 0.5 mgl-1 IAA alone. Plant tissue culture techniques have been applied
to produce disease-free planting materials of black ginger to overcome these problems.
Hence, the in vitro induced microrhizomes are considered as alternative disease-free
planting materials for black ginger cultivation. Therefore, this study was conducted
to optimize sucrose and plant growth regulators (PGRs) for its microrhizome induction.
Microrhizomes were successfully induced in Murashige and Skoog (MS) medium supplemented
with a high sucrose concentration 75 gml-1sucrose). NAA at 1.4 mgl-1 was found more
effective for microrhizome diameter, fresh and dry weight. The proliferated shoots
were green and healthy in appearance. Finally, healthy and complete plants with
well-developed roots were hardened, acclimatized and planted in the field successfully
with a survival rate of 100%.
