Effect of NAA
and picloram on the callus induction in Aloe barbadensis
Chandoliya Rakhi, Sharma Shivika, Joshi Rohit and Sharma Vikas
Res. J. Biotech.; Vol. 20(3); 209-212;
doi: https://doi.org/10.25303/203rjbt2090212; (2025)
Abstract
Aloe barbadensis miller is the most important medicinal plant used globally in the
cosmeceutical, pharmaceutical and nutraceutical industries. This present study investigates
the effect of different plant growth regulators on the in vitro callus induction.
The leaf explant was dissected from the 1-month plant and inoculated in the MS media
containing NAA (0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 4 mg/l) and picloram (0.5, 1.0, 1.5,
2.0, 2.5, 3.0 mg/l) either alone or in combination with BAP and kinetin.
Morphological parameters like color, texture and biomass of the callus were recorded
after 50 days. Maximum callus induction was obtained on the MS media supplemented
with 4mg/l picloram and 3mg/l NAA+0.5 mg/l kinetin. This innovated method could
be utilized as a reference for the large-scale propagation of medicinal plant Aloe
barbadensis.
