Fibrinolytic Enzyme
from Bacillus Pumilus Strain (MPMBJ1) through Solid-State Fermentation
Gnanaselvan Suvathika, Pandiyan Balamurugan, Devaraj Dhivya, Balasubramanian Haritha
and Sangilimuthu Alagar Yadav
Res. J. Biotech.; Vol. 20(3); 168-176;
doi: https://doi.org/10.25303/203rjbt1680176; (2025)
Abstract
Fibrinolytic enzymes are produced by several Bacillus species. For the therapeutic
purpose, the fibrinolytic enzyme can be used as thrombolytic agent for the clot
resolution. In the study, fibrinolytic enzymes are produced in the modified nutrient
medium under solid-state fermentation using Bacillus pumilus strain-MPMBJ1 from
CBNR isolate. The presence of fibrinolytic enzyme in the fermentation media was
confirmed by salting-out methods in conjunction with SDS-PAGE. The enzyme was purified
by using dialysis method followed by the chromatographic technique. Biological activities
of the enzyme were assessed through proteolytic and fibrinolytic functional assays.
The results showed that the optimum incubation period for fibrinolytic enzyme production
is 24 hrs. We have obtained the yeilds of fibrinolytic enzyme as 64.55 ± 1.25 and
22.75 ± 0.75 mg/ml by using acetone and ammonium sulfate precipitation methods respectively.
The molecular weight of the fibrinolytic enzyme was found to be 30kDa from SDS-PAGE
analysis. The enzyme was found to exhibit potential proteolytic and fibrinolytic
properties. Hence, further in vivo and clinical analysis on this enzyme may lead
to the production of an effective drug for heart attack patients.
