Phytochemical
Screening and Antimicrobial activity of Tephrosia villosa
Loka Chandrudu P., Ramesh Londonkar L., Shirisha Kumari S., Revansiddappa S.G.,
Jyothi K., Swetha C.M. and Jayachandra S.Y.
Res. J. Biotech.; Vol. 20(3); 118-124;
doi: https://doi.org/10.25303/203rjbt1180124; (2025)
Abstract
Phytochemical screening, antibacterial activity and anti-inflammatory properties
of Tephrosiavillosa root, stem and leaves were assessed in this study. Phytochemical
screening of the plant extracts (root, stem and leaf) of Tephrosia villosa was made
by running the root, stem and leaf through solvent such as ethanol. The ethanolic
extract of root indicated positive tests for steroids, alkaloids, amino acids and
proteins, saponins, flavones, anthocyanines and phenolic compounds. Ethanolic extract
of stem and leaf indicated positive tests for steroids, alkaloids, carbohydrates,
glycosides, amino acids and proteins, saponins, flavones, anthocyanines and phenolic
compounds. Ethanolic extracts of root, stem and leaves tested for their antibacterial
activity, the extracts in each series indicate modest to higher activity against
both Gram negative and Gram positive pathogenic microorganisms’.
The leaves extract of Tephrosia villosa indicated promising activity against the
dangerous pathogens such as K. pneumonia, Salmonella typhi and Salmonilla paratyphi,
by producing 13mm, 13mm and 12mm zone of inhibition around the inoculation. Plant
root extract was effective against the Staphylococcus aureus (12mm), Salmonella
typhi (12mm) and Salmonilla paratyphi (11mm).
