Research Journal of Biotechnology

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Application of NAA and BA for improving the yield along with flavonoid and polyphenol content of Eleutherine bulbosa bulb

Tran Thi Thanh Hien

Res. J. Biotech.; Vol. 20(5); 55-60; doi: https://doi.org/10.25303/205rjbt55060; (2025)

Abstract
Eleutherine bulbosa (Mill.) urb. is a herbaceous bulbous plant, known for its high medicinal value. To improve bulb yield and quality, controlling bulb growth as well as flavononoid and polyphenol content by NAA and BA was the objective of this study. Apply NAA and BA separately at different concentrations (BA: 5 ppm, 10 ppm and 15 ppm; NAA: 5 ppm, 10 ppm and 15 ppm) on E.bulosa at 6 weeks of age. After 4 weeks of treatment, tuber diameter, fresh weight, dry weight, number of leaf sheaths, total sugar content, starch, polyphenol and flavonoid in bulb were determined. Then, combined treatment with NAA 10 ppm and BA 10 ppm (at weeks 6 and 10), after 6 weeks of treatment, bulb fresh weight, dry weight, flavonoid and polyphenol content and bulb yield per plant were determined. The results showed that the 10 ppm BA treatment had the highest bulb diameter, fresh weight, dry weight, polyphenol content compared to other treatments and controls, while the 10 ppm NAA treatment had the highest flavonoid content but dry weight and bulb diameter were lower than the 10 ppm BA treatment.

In all the treatments, the number of leaf sheaths was not different from that of the control. In the combined treatments, bulb fresh weight, dry weight and polyphenol content and bulb yield per pot were significantly increased in the BA (w6) + BA (w10) treatment, slightly increased compared to the control and lower than other treatments. In the treatment of NAA (w6) + NAA (w10), it had a high flavonoid content but the lowest polyphenol content compared to other treatments. The relationship between sugar, starch, polyphenol and flavonoid content is also discussed.