Assessment of
Mycochemical composition and biological activities of extracts from Humphreya endertii
Bao-Anh Trịnh, Hanh Thi-Dieu Nguyen, Ngoc-An Nguyen, Thao Nguyen-Phuong Pham and
Tan-Viet Pham
Res. J. Biotech.; Vol. 20(5); 135-141;
doi: https://doi.org/10.25303/205rjbt1350141; (2025)
Abstract
This study presents the comprehensive analysis of the chemical composition and biological
activities of methanol and ethanol extracts from Humphreya endertii, a recently
discovered mushroom species in Vietnam. Gas chromatography/mass spectrometry identified
16 compounds in the methanol extract and 20 in the ethanol extract, with significant
differences in composition between the two. The methanol extract contained 18.51%
5-Methoxypyrrolidin-2-one, 15.41% cis-Vaccenic acid, 14.03% 10(E),12(Z)-Conjugated
linoleic acid, 13.95% n-Hexadecanoic acid, 8.25% 2,5-Furandione, dihydro-3-methylene
and other compounds in smaller amounts (<8%). The ethanol extract was composed of
31.30% 17-Octadecynoic acid, 23.50% cis-Vaccenic acid, 16.32% n-Hexadecanoic acid,
10.59% Octadecanoic acid, 5.99% Ethyl α-d-glucopyranoside and other compounds in
smaller amounts (<5%).
Both extracts exhibited antibacterial activity against ten bacterial pathogens including
S. aureus, E. hormachei, E. faecalis, B. cereus, S. sonnei, P. aeruginosa, S. typhimurium,
S. saprophyticus, E. cloacae and S. enteritidis. Antioxidant properties were also
observed through DPPH radical scavenging activity, with similar IC50 values of approximately
635 μg/mL for both the methanol and ethanol extracts. These findings suggest that
the methanol and ethanol extracts of H. endertii could be valuable for pharmaceutical
applications and the development of medical resources in the future.
