Induction and
identification of polyploidy by colchicine treatment in balloon flower (Platycodon
grandiflorus)
Pham Dieu Thi, Le Thuy Huong, Luu Tu Thi Thanh and Huynh Thao Thanh
Res. J. Biotech.; Vol. 20(5); 61-67;
doi: https://doi.org/10.25303/205rjbt61067; (2025)
Abstract
Balloon flower (Platycodon grandiflorus) is a valuable medicinal plant in traditional
medicine. Experiments on shoot formation and rapid multiplication have been conducted
to create a source of mutation-inducing material. The Murashige and Skoog (MS) medium
supplemented with 1.0 mg/l BAP; 0.4 mg/l BAP and 0.1 mg/l NAA showed good responses
in shoot formation and rapid multiplication of shoots. The shoot clusters were immersed
in different concentrations of colchicine solutions (0%; 0.01%; 0.02%; 0.03%; 0.04%;
w/v) for 24, 48 and 72h.
The results indicated that treatment at 0.02% colchicine for 48h had the highest
polyploid shoot formation induction. The polyploid shoots exhibited lager stomatal
size and denser distribution. The ploidy levels of polyploid shoots were assessed
by counting number chromosomes and by flow cytometry.
