Isolation of putative
geranylgeranyl pyrophosphate synthase type III gene from Rhodosporidium toruloides
VN1
Chung Hue Huan and Dai-Nghiep Ngo
Res. J. Biotech.; Vol. 20(5); 201-205;
doi: https://doi.org/10.25303/205rjbt2010205; (2025)
Abstract
Carotenoids are valuable pigments with significant applications in industry, thus,
the demand for these natural compounds is increasing, especially carotenoids from
natural sources. Rhodospordidium toruloides, a red yeast chassis, has the ability
to produce carotenoids in high content. But the functions of many genes involved
in the carotenoids biosynthesis pathway in this yeast remain poorly understood.
In this research, genomic DNA from R. toruloides VN1 was successfully extracted
through an SDS-proteinase K method.
The DNA was then used to amplify the putative geranylgeranyl pyrophosphate synthase
gene type III (crtE), the product was sequenced using Sanger method and this sequence
was cloned into an Escherichia coli strain by a recombinase system for further investigation.
