The effects of
phycocyanin to relieve oxidative damage on mouse dermal papilla cells
Minh Quan To, Ngoc Cam Giang Bui, Thi Thao Van Pham, Hoang Ngoc Ngan Trang and Thanh
Long Le
Res. J. Biotech.; Vol. 20(5); 159-167;
doi: https://doi.org/10.25303/205rjbt1590167; (2025)
Abstract
The reactive oxygen species (ROS) is one of the main reasons leading to the senescence
and apoptosis of dermal papilla cells, whose function is to regulate the hair cycle.
Phycocyanin (CPC), a pigment-protein, is a powerful antioxidant that can stimulate
fibroblasts' and keratinocytes' proliferation. This study experimented with the
ability of CPC to reduce oxidative stress. CPC was extracted from Arthrospira platensis
and was purified. Mouse dermal papilla cells (DP cells) were isolated from the vibrissa
of C57/BL6 mouse and cultured by explant method. The DP cells were pre-treated with
CPC (5, 10 μg/ml) and 150 μM H2O2 to test their protective ability.
The results showed that CPC was collected with analytical grade (PI=4.11). The cultured
cells expressed some DP cells' main characteristics: fibroblast-like shape, vimentin,
α – SMA, formation of cell clusters and spheroid in 3D culture. CPC is non-toxic
to DP and 5-10 μg/ml concentration induced DP proliferation. The CPC 5-10 μg/ml
reduced H2O2-induced damage, decreasing death rate, total ROS and ß-galactosidase
expression, preserving cell morphology and inducing cell growth. CPC 10 μg/ml is
the optimal concentration that induces PD replication and alleviates H2O2-induced
oxidative stress on DP.
