The in vitro biocompatibility
and calcification evaluation of Xenogeneic vascular graft
Nho Thuan Nguyen, My Thi Ngoc Nguyen, Hoang Minh Lam and Ha Le Bao Tran
Res. J. Biotech.; Vol. 20(5); 40-44;
doi: https://doi.org/10.25303/205rjbt40044; (2025)
Abstract
Xenogenic tissues, including pericardium and vascular vessels have been researched
and used in the cardiovascular surgery. The Xenogenic tissues were treated with
glutaraldehyde to reduce antigenicity and to improve strength and durability. Tissue
derived extracellular matrix (ECM) has been shown to support host tissue remodeling
and integration. However, calcification due to glutaraldehyde remains. Therefore,
the field of cardiovascular patch fabricated from bovine pericardium is still being
studied. In our previous research, the acellular bovine pericardium was treated
with low glutaraldehyde concentration (so-called the modified pericardium). The
modified pericardial membranes were investigated regarding in vitro biocompatibility
toward endothelial cells and calcification effect.
It was demonstrated that the modified pericardium did not cause cell cytotoxicity
and supported endothelial cell adhesion on the membrane surface. The in vitro and
in vivo analysis presented a significantly low level of calcification, which was
shown via a decrease in vitro calcium content accumulation and the absence of in
vivo mineralized area. These results indicate the modified pericardium's potential
for use as a cardiovascular graft.
