A comprehensive
study of tuna dry fish protein hydrolysates (DFPH) via papain-mediated hydrolysis
and fermentation with Lactobacillus delbrueckii
Mariam Nooru K., Samson Lijoseraj C., Sarah Sundar Blessy and Premina S.
Res. J. Biotech.; Vol. 20(11); 205-213;
doi: https://doi.org/10.25303/2011rjbt2050213; (2025)
Abstract
Tuna dry fish demonstrates remarkable potential in nutraceutical applications due
to its high content of essential proteins and bioactive compounds. This research
examines the bioactive characteristics of fermented tuna dry fish protein hydrolysate
(DFPH) which was produced through enzymatic papain hydrolysis followed by Lactobacillus
delbrueckii fermentation. The bioactivity assessment of both fermented and non-fermented
DFPH involved antioxidant testing using DPPH radical scavenging evaluation and inflammatory
response testing via albumin denaturation inhibition analysis alongside MTT testing
against HCT 116 colorectal cancer cells to determine anticancer activity.
This research demonstrates that fermenting DFPH led to enhanced bioactive properties
resulting in improved radical scavenging ability at IC₅₀ = 107.91 μg/mL and enhanced
anti-inflammatory capacity at IC₅₀ = 198.06 μg/mL along with cytotoxicity against
cancer cells. The bioactive compounds identified by GC-MS analysis within fermented
DFPH showed antioxidant, anticancer and anti-inflammatory properties. This study
highlights that fermentation processes improve bioactive features of tuna dry fish
hydrolysates making them suitable for food production and pharmaceutical applications.
