Evaluation of
phytochemicals, in vitro antioxidant and antidiabetic activities of Aquilaria malaccensis
Lam.
Kalita Partha Pratim, Saikia Surovi and Kashyap Bhargav
Res. J. Biotech.; Vol. 20(11); 258-263;
doi: https://doi.org/10.25303/2011rjbt2580263; (2025)
Abstract
The study focuses on the evaluation of polyphenols, in vitro antioxidant and antidiabetic
activities of methanolic extract from the leaves of Aquilaria malaccensis Lam, collected
from Assam, India. The leaves underwent 70% methanolic extraction, followed by analysis
of total phenolic content (TPC) using the Folin-Ciocalteu method and total flavonoid
content (TFC) via the aluminum chloride colorimetric method. The study aimed to
investigate the potential bioactive properties of polyphenols present in the plant
extract. The antioxidant capacity was assessed through the DPPH (1,1-diphenyl-2-picrylhydrazyl)
radical scavenging assay and the antidiabetic potential was evaluated using the
α-amylase inhibitory assay. Phytochemical analysis confirmed the presence of polyphenols,
flavonoids, saponins, tannins and terpenes, highlighting the rich phytochemical
profile of the extract.
The results showed that the methanolic extract had a TPC of 66.26 ± 0.667 μg GAE/mL
and a TFC of 134.85 ± 0.776 μg/mL. The extract demonstrated antioxidant activity
with a maximum inhibition of 74.23% at 200 μg/mL. In terms of antidiabetic activity,
the extract achieved 78.24% inhibition of α-amylase activity at 600 μg/mL. These
findings suggest that the Aquilaria malaccensis Lam. leaves possess antioxidant
and antidiabetic properties, positioning the plant as a potential source for natural
therapeutic agents against oxidative stress and diabetes.
