Pharmacognostic
standardization and HPTLC fingerprint profile of Careya arborea Roxb. stem bark
Gupta Priyanka, Patil Darshana and Patil Avinash
Res. J. Biotech.; Vol. 20(11); 293-299;
doi: https://doi.org/10.25303/2011rjbt2930299; (2025)
Abstract
To ensure reproducible quality of herbal products, proper control of raw material
is important. The first step towards ensuring quality of starting material is pharmacognostic
standardization. Thus, in recent years, there has been a rapid increase in the standardization
of selected medicinal plants of potential therapeutic significance. Careya arborea
Roxb. is one of the important medicinal plants belonging to family Lecythidaceae.
The present work has been designed to evaluate the pharmacognostic and phytochemical
parameters of the stem bark of Careya arborea Roxb for the standardization. Various
standardization parameters like macroscopic characters, microscopic evaluation,
fluorescence analysis, histochemical study, physicochemical evaluations, preliminary
phytochemical screening were carried out according to Ayurvedic Pharmacopeia of
India (API) and WHO guidelines and the qualitative parameters were reported. HPTLC
fingerprint profile was also evaluated by standard method.
Microscopy of the bark showed the presence of different type of calcium oxalate
crystals, stone cells, sclerids and lignified fibres. Total ash value, acid soluble
ash, water soluble ash, alcohol soluble extract, water soluble extract, loss on
drying and swelling index were found to be 12.03 ± 0.25, 0.8 ± 0.002, 8.43 ± 0.012,
23.88 ± 0.01,21.96 ± 0.02,7.82 ± 1.56,3.33 ± 0.47 respectively. Phytochemical analysis
of different extracts showed the presence of aleurone grains, carbohydrates, fats
and fixed oils, mucilage, starch, alkaloids, glycosides, saponins, anthraquinones,
essential oils, tannins, steroids, triterpenoids and flavonoids. These studies suggested
that the observed pharmacognostic, physicochemical and phytochemical parameters
provide referential information for correct identification, authentication of the
plant material, formulation development.
