Unlocking the
potential of Vibrio natriegens for L-lysine production
Nghiem Thi Tuoi, Tran Huong Ly, Nguyen Tien Thanh, Le Thanh Ha and Le Quang Hoa
Res. J. Biotech.; Vol. 20(11); 1-9;
doi: https://doi.org/10.25303/2011rjbt0109; (2025)
Abstract
L-lysine is an essential amino acid widely used for feed additive and pharmaceutical
purposes. It is produced via microbial fermentation, mainly using Corynebacterium
glutamicum. Recently, Vibrio natriegens has become a promising host in the bioindustry,
thanks to its rapid growth, versatility in substrate utilization and high salt-tolerance.
The aim of this study is to evaluate the feasibility of L-lysine production in V.
natriegens.
Introduction of six genes encoding core metabolic enzymes and transporter involved
in lysine biosynthesis from C. glutamicum into V. natriegens genome and deletion
of endogenous pckA gene enabled the production of L-lysine with a titer of 4.6 g/L
(nearly 80-fold increase in comparison with the wild-type) and a yield of 152 mmol/mol
glucose in the defined synthetic medium after 40 hours of fermentation in shake
flask. We reported the feasibility of L-lysine biosynthesis in V. natriegens.
