Screening of antibacterial
producing endophytic bacteria isolated from Medicinal plant Coleus amboinicus
Thenmozhi Panneerselvam, Chelladurai Iyyanar, Ayyanar Pandurangan, Sugumaran Pichamuthu
and Gajalakshmi P.
Res. J. Biotech.; Vol. 20(9); 127-133;
doi: https://doi.org/10.25303/209rjbt1270133; (2025)
Abstract
The objective of the current study was isolation of endophytic bacteria from Coleus
amboinicus L. leaves and to evaluate its metabolite efficacy. Totally 5 bacterial
colonies were isolated by direct plating method of which three strains are Gram
positive in nature. Among 5, three were oxidase positive and all are catalase positive
in nature. Isolate strain (CA2) is endospore producing rod bacterium identified
as Bacillus sp showing positive antibacterial effect on test pathogens. The antimicrobial
potential of endophytes reveals that isolate Bacillus sp has potent promising antibacterial
agent production against Escherichia coli, Proteus vulgaris, K.pneumoniae and Candida
albicans. The strain antibiotic production is largely regulated by dextrose, moderately
by fructose and lactose and not by sucrose and sodium acetate. The compound is isolated
and metabolite profile is identified by GCMS revealing 45 peak and 40 different
compounds with important pharmacologically known active molecules.
Strychane, 1-acetyl-20.alpha.-hydroxy-16-methylene, photocitral B, 2-[2-(Benzoyloxy)ethoxy]ethyl
benzoate,3-nonanone, guanethidine and acebutolol were used for antimycobactrial
drug screening by in silico against 3-Oxoacyl-[acyl-Carrier protein] Reductase.
Molecular docking reveals that acebutolol and guanethidine are strong antimycobaterial
agents. In conclusion, this study demonstrated that medicinal plants had a wide
variety of cultivable endophytic bacteria and serve as a promising source for the
production of industrially important organic acids.
