GC-MS Analysis
and Bioactivity Assessment of Protonated Alstonia macrophylla Methanolic Extract
against Oxidative Stress, Inflammation and Cervical Cancer Cells
Vijayan Sobana, Olaganathan Sivapriya and Muruganantham Sankareswaran
Res. J. Biotech.; Vol. 21(2); 185-199;
doi: https://doi.org/10.25303/212rjbt1850199; (2026)
Abstract
The methanolic leaf extract of Alstonia macrophylla was used in vitro tests to examine
its anti-inflammatory, anti-cancer and antioxidant properties. GC-MS analysis revealed
that the extract's primary components were fatty acid derivatives, gamma-sitosterol
(11.27%) and thymol (53.7%). It also demonstrated modest DPPH radical scavenging
action (IC₅₀ = 178.34 μg/mL). Anti-inflammatory assays demonstrated dose-dependent
inhibition of albumin denaturation (IC₅₀ = 214.56 μg/mL) and HRBC membrane stabilization
(IC₅₀ = 247.34 μg/mL), though less potent than aspirin.
Notably, the extract showed potent cytotoxicity against HeLa cervical cancer cells
(IC₅₀ = 6.04 μg/mL), with complete inhibition at 1000 μg/mL, likely mediated by
indole alkaloids. These findings validate A. macrophylla's traditional uses and
demonstrate its potential as a treatment for inflammation, cervical cancer and illnesses
associated with oxidative stress. Additional research is necessary to identify active
ingredients and clarify mechanisms of action.
