Simple and easy
method to isolate nucleic acids and protein from leaf sample
Hazarika Pranita, Singh H. Ranjit, Das Dhiraj Kumar, Ali Cintom and Das Sudripta
Res. J. Biotech.; Vol. 21(2); 137-152;
doi: https://doi.org/10.25303/212rjbt1370152; (2026)
Abstract
Here we report about the standardization of simultaneous extraction of nucleic acids
and proteins from leaf samples. The protocol established a simple, user-friendly
and affordable method to isolate DNA, RNA and protein from a single leaf sample
suitable for genomic and proteomic studies. The result showed that the extracted
biomolecules were qualitatively and quantitatively unaffected at the end of the
extraction procedure. The protocol was successfully tested in thirty-three different
leaf samples covering annual, biennial and perennial plants. It took three hours
to complete the protocol. Nucleic acids and proteins were successfully used for
downstream application.
The extracted DNA molecules were tested for restriction digestion with EcoRI and
HindIII, gene cloning and molecular marker analysis with SSR marker. Complementary
DNA synthesis, gene cloning and gene expression analysis were done by taking isolated
total RNA molecules. Protein profiling was done by performing SDS-PAGE and Q-TOF
MS analysis.
