Soybean Seed Extract
induced Inhibition of Jab1 in Breast Cancer Cell Line
Nandakumar Vidya, Gunasangkaran Gayathiri, Muthukrishnan Saradhadevi, Arumugam Vijaya
Anand and Muthukrishnan Arun
Res. J. Biotech.; Vol. 21(2); 71-80;
doi: https://doi.org/10.25303/212rjbt071080; (2026)
Abstract
Scientific research has suggested that the modification of signaling networks has
a strong relationship with the progression of breast cancer and alterations by phytocompounds
could offer a promising approach for treating and managing the disease. Therefore,
we evaluated the inhibitory potency of soybean seed extract against Jab1 in breast
cancer using several in vitro assays. Our findings revealed that treatment with
soybean seed extract in breast cancer cells resulted in a dose- and time-dependent
reduction in cell growth, with an IC50 value of 67.68 μg/ml. Furthermore, the antioxidant
activity of the soybean seed extract was confirmed by the DPPH assay. Additionally,
soybean seed extract-treated triple-negative breast cancer cells exhibited increased
nuclear condensation, indicating apoptosis.
Real-time PCR results demonstrated the cell growth inhibitory potential of the soybean
seed extract by downregulating Jab1 expression. At the same time, there was a significant
increase in Bax and caspase 3 expression, which suggests cell cycle arrest at the
G0/G1 phase. Moreover, increased caspase 3 activation elucidates one of the possible
mechanisms behind the inhibitory effect of soybean seed extract against Jab1 in
breast cancer cells (MDA-MB-231). In conclusion, our study suggests that the bioactive
compounds in soybean may serve as potent therapeutic agents for targeting the oncogene
Jab1 in breast cancer.
