Genetic Analysis
of Dengue Fever - A Comprehensive Investigation of Associated Genes and their Functional
Pathways
Bonam Venkata Ramesh, Sampara Vasishta, Adiga Usha and Tunuguntla Amulya
Res. J. Biotech.; Vol. 21(1); 320-329;
doi: https://doi.org/10.25303/211rjbt3200329; (2026)
Abstract
Dengue fever represents a significant global health challenge with complex pathophysiological
mechanisms. This study aimed to elucidate the genetic underpinnings of Dengue fever
through comprehensive analysis of associated genes. The top 30 genes related to
Dengue fever were retrieved from the DisGeNET database and analyzed using multiple
bioinformatics tools including GO terms (Biological Process, Cellular Component,
Molecular Function), Wiki Pathways and Jensen databases. R version 4.4.2 was employed
for data analysis and visualization. Analysis revealed significant enrichment of
genes involved in cytokine production regulation, particularly interleukin-6 and
inflammatory response pathways. Key biological processes included STAT protein phosphorylation
regulation (OR=66.37, p<0.001) and nitric oxide biosynthesis (OR=133.42, p<0.001).
Cellular localization identified extracellular and immune-related compartments as
primary sites, while molecular function analysis highlighted cytokine activity (OR=42.35,
p<0.001).
Pathway analysis demonstrated significant associations with folate metabolism (OR=168.74,
p<0.001) and cytokine inflammatory responses (OR=319.58, p<0.001). Tissue analysis
revealed predominant expression in immune-related tissues, particularly the peritoneal
cavity (OR=950.52, p<0.001). This study elucidates the complex genetic landscape
underlying Dengue fever pathogenesis, highlighting the central role of inflammatory
cytokine regulation, particularly IL-6, IFNG, TNF and IL-1B in disease manifestation.
These findings provide potential targets for therapeutic intervention and diagnostic
biomarker development for improved management of Dengue fever.
