Metabolomic Profiling
and Hypoglycemic Potential of Spirulina platensis Extracts of Infusion and Decoction
Techniques through in silico α-Amylase and α-Glucosidase Inhibition
Firdaus Muhamad, Yahya Yahya, Priambodo Ahmad Faris and Gaisani Rizqia Zuhri Arifa
Res. J. Biotech.; Vol. 21(1); 338-343;
doi: https://doi.org/10.25303/211rjbt3380343; (2026)
Abstract
Spirulina platensis contains a group of metabolomes that can inhibit glucose absorption.
This study aimed to identify metabolites in the infusion and decoction of S. platensis
and evaluate their ability as inhibitors of α-amylase and α-glucosidase through
the in silico method. The samples were analyzed using HPLC-HRMS by untargeted method
for metabolite identification while molecular docking studies were conducted using
PyRx 0.8 and physicochemical properties were analyzed using SwissADME. The results
showed 120 metabolites identified, with 84 from decoction and 67 from infusions.
Molecular docking studies revealed that zearalenone and adenosine have a strong
binding affinity for α-amylase and α-glucosidase, with an affinity energy of -8.6
kcal/mol, lower than that of acarbose.
Zearalenone forms a hydrogen bond with Ile230 residue on α-amylase while adenosine
forms a hydrogen bond with His332 and Arg400 residues on α-glucosidase. Analysis
of physicochemical properties according to Lipinski's rule shows that both compounds
meet the criteria for good bioavailability. Further molecular and in vivo dynamics
studies are needed to strengthen the results of this research.
