Simple and eco-friendly
magnetite preparation, its characterization and DNA extraction application
Tjoa Stanley Evander Emeltan, Mudasir Mudasir, Suharyadi Edi and Daryono Budi Setiadi
Res. J. Chem. Environ.; Vol. 29(4); 1-6;
doi: https://doi.org/10.25303/294rjce0106; (2025)
Abstract
Magnetite nanoparticles in this study were synthesized using a simple and eco-friendly
method. The synthesized particles were characterized using a Scanning electron microscope
(SEM), Energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and Vibrating
sample magnetometry (VSM). The ability of the particles to interact with DNA was
evaluated using Infrared spectrophotometry (IR) and tested in DNA extraction procedures
for several microbial species. Results showed that synthesized magnetite exhibited
an agglomerated structure with nanoscale dimensions. Fe and O elements, detected
through EDS analysis, confirmed the formation of iron oxide during the synthesis
process. Magnetite characteristics were demonstrated in the XRD analysis, with the
highest intensity at 2θ value 35.45º, interplanar spacing 2.530 Å and crystallite
size of 17.422 nm.
The magnetic property of the synthesized magnetite was identified as ferromagnetic.
The interaction between the magnetite and DNA was evident at 944.63, 1103.79, 1124.97
and 1161.37 cm⁻¹ in the IR spectra. The produced magnetite was successfully applied
for microbial DNA extraction from a laboratory consortium including E. coli, P.
aeruginosa, S. aureus, S. paratyphi and M. tuberculosis. All extracted DNA samples
were successfully amplified using a 16S rRNA primer pair. This demonstrated ability
to extract microbial DNA makes the synthesized magnetite suitable for environmental
monitoring applications including microbial identification and contamination detection.
