Electrochemical
Resolution of Ascorbic Acid from Dopamine and Uric Acid with Montmorillonite k10-Clay/Poly
(Glycine) Electrode
Prabhakar Rao V., Jeevan Jyoti P., Bhanuprakash P. and Miditana Sankara Rao
Res. J. Chem. Environ.; Vol. 29(2); 45-54;
doi: https://doi.org/10.25303/292rjce045054; (2025)
Abstract
Ascorbic acid is a physiologically important biomolecule, acting as an antioxidant
and controlling conditions like scurvy, cancer and AIDS. Hence, it is crucial to
estimate its levels in body fluids to detect any disorders. In this study, a cost-effective
cyclic voltametric method was used to determine ascorbic acid in the presence of
uric acid and dopamine. A new modified carbon paste electrode, namely Montmorillonite
K10-clay/Poly, was fabricated for this purpose. The obtained modified electrode
exhibits excellent electrocatalytic activity for the selective detection of ascorbic
acid, even in the presence of uric acid and dopamine. Its sensitivity for ascorbic
acid is doubled compared to the bare carbon paste electrode.
The detection limit and quantification limit of this electrode for ascorbic acid
are found to be 1.13×10-6 M and 3.77×10-5 M respectively. Additionally, the modified
electrode recovers 96.5% of ascorbic acid in real sample analysis, such as the analysis
of a vitamin C tablet (Celin-500mg). The selectivity, reproducibility and sensitivity
of this modified electrode provide a promising approach for applying the technique
in routine analysis of selected electroactive biomolecules in clinical and bioanalytical
laboratories.
