Synthesis, Spectral
Characterization, in vitro cytotoxicity and antibacterial studies of a Novel Schiff
Base Ligand derived from S-Carboxy Methyl-L-Cysteine and 5-Chloroisatin and its
Cu(II), Ni(II) and Co(II) Metal complexes
Lisandra N.R., Deena Antony C. and Saritha V.S.
Res. J. Chem. Environ.; Vol. 29(7); 12-19;
doi: https://doi.org/10.25303/297rjce012019; (2025)
Abstract
A novel chiral Schiff base ligand, namely S-Carboxy Methyl-L-Cysteine 5-Chloroisatin
(CMCCI) was synthesized through the condensation of the chiral amino acid S-Carboxy
Methyl-L-Cysteine and 5-Chloroisatin in basic medium. The resulting amino acid Schiff
base ligand and its metal complexes with Cu(II), Ni(II) and Co(II) were characterized
using elemental analysis, FTIR, 1H NMR and UV-visible spectroscopy, confirming the
formation of CMCCI and (CMCCI)2M type metal complexes. The formation of the ligand
CMCCI was also confirmed by mass spectral analysis. The analytical results suggested
that the Schiff base behaves as tridentate ONO donor ligand and coordinates with
Cu(II), Ni(II) and Co(II) ions in octahedral geometry.
The ligands and their complexes were screened for their cytotoxicity and antibacterial
activities. Cytotoxicity studies revealed that the Schiff base complexes of copper
exhibited considerable cytotoxic effects than the Schiff base ligand. Copper complex
showed high cytotoxicity even in lower concentrations and can be used as a potential
anticancer drug after further studies. Antibacterial studies of the ligand and metal
complexes were performed against Staphylococcus aureus and Escherichia coli, Gram
positive and Gram negative bacterial strains respectively. Copper complex exhibited
considerable activity against Staphylococcus aureus and cobalt complex showed moderate
activity against Escherichia coli.
